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Standard reaction buffer for PCR using FastStart Taq DNA Polymerase. Application Use this buffer together with FastStart Taq DNA Polymerase. For applications refer to FastStart Taq DNA Polymerase, 5 kU, GMP Grade. Specifications Appearance: Clear, colorless solution
action of homogenous human recombinant endoG on DNA and chromatin substrates. We have found that the enzyme pos-sesses novel properties including cooperation with exonuclease and DNase I for more efficient DNA breakdown under physio-logical ionic strengths. EXPERIMENTAL PROCEDURES Expression and Purification of Recombinant EndoG—Full-length hu-
A targeted regional-mapping approach and a target-capturing assay were used to identify the binding region on the aptamer molecule. In the detection assay, referred to as “aptamer-based regionally protected PCR” (ARP-PCR), the aptamer was allowed to bind to the target protein in solution before digestion with DNase I.
ABSTRACT: Nebulized recombinant human deoxyribonuclease (DNase) reduces sputum viscosity and improves lung function in some cystic fibrosis patients, but individual responses are unpredictable. The aim of this study was to investigate how DNase can be targeted to those cystic fibrosis patients who would benefit most.
Complete Protease Inhibitor Cocktail, Roche) 14. Lysozyme (Sigma) (300 mg/ml) 15. DNase I (Roche Applied Science, 1 mg/ml) þ MgCl 2 16. High salt wash buffer (lysis buffer containing 1 M NaCl) 17. Elution buffer (lysis buffer þ 250 mM Imidazole, pH 8.0) 18. Ni NTA resin (Qiagen) 19. Millipore Amicon Ultra-4 centrifugal filter units, 10 kDa
The ability of recombinant human DNase I (DNase I) to degrade DNA to lower molecular weight fragments is the basis for its therapeutic use in cystic fibrosis patients (1). It is in-haled into the
Enzyme acivities: Highly processive 5′-3′ DNA polymerase; double-strand specific 5′-3′ exonuclease; no 3′-5′ exonuclease activity Heat activation: +95°C for 3-10 minutes (assay-dependent; recommendation is 10 minutes for full activation) pH optimum: Approximately 9.0 (+25°C) Temperature optimum: Approximately +75°C
A, 40 g of DNA purified from nuclei treated with either DNase I (15 and 30 min) or purified recombinant endoG (20 and 40 min) was separated on a sequencing gel. B, purified mononucleosomal DNA
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Recombinant human deoxyribonuclease I (DNase I) is an important clinical agent that is inhaled into the airways where it degrades DNA to lower molecular weight fragments, thus reducing the viscoelasticity of sputum and improving the lung function of cystic fibrosis patients. To investigate DNases with potentially improved properties, we constructed a molecular fusion of human DNase I with the
Dornase alfa (proprietary name Pulmozyme from Genentech) is a highly purified solution of recombinant human deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA.Dornase alfa hydrolyzes the DNA present in sputum/mucus of cystic fibrosis patients and reduces viscosity in the lungs, promoting improved clearance of secretions. . This protein therapeutic agent is produced in DNase I（Deoxyribonuclease I）中文名称为 脱氧核糖核酸酶 I，是一种可以消化单链或双链DNA产生单脱氧核苷酸或单链或双链的寡脱氧核苷酸的 核酸内切酶 。. DNase I水解单链或双链DNA后的产物，5’端为磷酸基团，3’端为羟基。. DNase I活性依赖于钙离子，并能被镁离子或二价锰离子激活。. 镁离子存在条件下，DNase I可随机剪切双链DNA的任意位点；二价锰离子存在条件下，DNase I可在
Dornase alfa (proprietary name Pulmozyme from Genentech) is a highly purified solution of recombinant human deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA.Dornase alfa hydrolyzes the DNA present in sputum/mucus of cystic fibrosis patients and reduces viscosity in the lungs, promoting improved clearance of secretions. . This protein therapeutic agent is produced in DNase I（Deoxyribonuclease I）中文名称为 脱氧核糖核酸酶 I，是一种可以消化单链或双链DNA产生单脱氧核苷酸或单链或双链的寡脱氧核苷酸的 核酸内切酶 。. DNase I水解单链或双链DNA后的产物，5’端为磷酸基团，3’端为羟基。. DNase I活性依赖于钙离子，并能被镁离子或二价锰离子激活。. 镁离子存在条件下，DNase I可随机剪切双链DNA的任意位点；二价锰离子存在条件下，DNase I可在
A formulation and process development study was performed to formulate recombinant human deoxyribonuclease I as a powder for inhalation. First, excipient compatibility (with bovine DNase as a